Johnson City is located at 36°18'47" North, 82°21'12" West (36.313056, -82.353611).
According to the United States Census Bureau, the city has a total area of 39.58 mi2 (102.51 km2). 39.27 mi2 (101.72 km2) of it is land and 0.31 mi2 (0.80 km2) of it is covered by water. The total area is 0.78% water.
Johnson City is located in the Eastern Standard Time (EST) timezone, GMT-05:00. It observes Daylight Saving Time (DST).