Ashwaubenon is located at 44°29'13" North, 88°4'59" West (44.487158, -88.083284).
According to the United States Census Bureau, the village has a total area of 12.74 mi2 (33.00 km2). 12.37 mi2 (32.04 km2) of it is land and 0.37 mi2 (0.96 km2) of it is covered by water. The total area is 2.92% water.
Ashwaubenon is located in the Central Standard Time (CST) timezone, GMT-06:00. It observes Daylight Saving Time (DST).