Lena is located at 44°57'2" North, 88°2'48" West (44.950833, -88.046667).
According to the United States Census Bureau, the village has a total area of 0.89 mi2 (2.30 km2). 0.88 mi2 (2.29 km2) of it is land and 0.00 mi2 (0.01 km2) of it is covered by water. The total area is 0.39% water.
Lena is located in the Central Standard Time (CST) timezone, GMT-06:00. It observes Daylight Saving Time (DST).